中文摘要：

天生的防御反應(yīng)雖然主要是本能的，但也必須高度適應(yīng)風(fēng)險(xiǎn)評(píng)估的變化。然而，在嚴(yán)重壓力之后，適應(yīng)性變化可能會(huì)變得適應(yīng)不良，如創(chuàng)傷后應(yīng)激障礙 （PTSD） 所見(jiàn)。在一系列實(shí)驗(yàn)中，我們觀察到雄性小鼠在不良休克經(jīng)歷后對(duì)先前無(wú)威脅的刺激的先天逃避行為的長(zhǎng)期變化表現(xiàn)為威脅反應(yīng)閾值的轉(zhuǎn)變。通過(guò)記錄上丘 （SC） 中的神經(jīng)活動(dòng)，同時(shí)對(duì)傳入神經(jīng)的特定反應(yīng)進(jìn)行光標(biāo)記，我們確定了從內(nèi)側(cè)前額葉皮層 （mPFC） 到達(dá) SC 的輸入對(duì)不良休克經(jīng)歷后逃逸相關(guān)活動(dòng)的直接和間接影響。在沖擊期間停用這些特定投影有效地消除了觀察到的變化。相反，在遭遇期間光遺傳學(xué)激活它們會(huì)控制逃逸反應(yīng)。這確定了那些特定的 mPFC 輸入到 SC 中對(duì)于先天逃脫行為的不良體驗(yàn)相關(guān)變化的必要性和充分性。

英文摘要：

Innate defensive responses, though primarily instinctive, must also be highly adaptive to changes in risk assessment. However, adaptive changes can become maladaptive, following severe stress, as seen in posttraumatic stress disorder (PTSD). In a series of experiments, we observed long-term changes in innate escape behavior of male mice towards a previously non-threatening stimulus following an adverse shock experience manifested as a shift in the threshold of threat response. By recording neural activity in the superior colliculus (SC) while phototagging specific responses to afferents, we established the crucial influence of input arriving at the SC from the medial prefrontal cortex (mPFC), both directly and indirectly, on escape-related activity after adverse shock experience. Inactivating these specific projections during the shock effectively abolished the observed changes. Conversely, optogenetically activating them during encounters controlled escape responses. This establishes the necessity and sufficiency of those specific mPFC inputs into the SC for adverse experience related changes in innate escape behavior.

論文信息：

論文題目：Prefrontal control of superior colliculus modulates innate escape behavior following adversity

期刊名稱：Nature Communications

時(shí)間期卷：15, Article number: 2158 (2024)

在線時(shí)間：2024年3月9日

DOI：doi.org/10.1038/s41467-024-46460-z

產(chǎn)品信息：

貨號(hào)1：R180

品名1：Red fluorescent tracer microspheres：Red Retrobeads™ IX

貨號(hào)2：G180

品名1：Green fluorescent tracer microspheres：Green Retrobeads™ IX

規(guī)格：100ul

品牌：Lumafluor

產(chǎn)地：美國(guó)

名稱：fluorescent tracer microspheres

現(xiàn)貨授權(quán)：Target Technology（靶點(diǎn)科技）

Lumafluor助力研究上丘的前額葉控制調(diào)節(jié)逆境后的先天逃逸行為文章截圖

Lumafluor助力研究上丘的前額葉控制調(diào)節(jié)逆境后的先天逃逸行為，產(chǎn)品使用截圖。

For anatomical tracing of SC afferents and DMS afferents, Green and Red fluorescent tracer microspheres (Retrobeads, Lumafluor) were injected into the SC (AP:?3.80?mm, ML:?±?0.8?mm, DV:?1.5?mm) and into the DMS (AP:?+?0.5?mm, ML:?±?1.25?mm, DV-3mm). The stock solution was diluted in pbs (1/4) and the volume per injection area was 600?μL.